Superoxide dismutases (SOD) are found in all aerobic organisms. Their physiological function appears to be to protect cells from free radicals by scavenging superoxide anions. They catalyse the dismutation of superoxide anions to oxygen and hydrogen peroxide. SOD(Mn) is located primarily in mitrochondria and is thought to be of prokaryote origin. SOD(Mn) is a tetramer of identical subunits of MW 25kD by SDS-PAGE.
Suitable for use in Western Blot, ELISA and Immunohistochemistry. Other applications not tested.
Western Blot: 1:2000
Immunohistochemistry (Formalin fixed paraffin embedded): 1:50
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.