Suppressors of cytokine signaling (SOCS) protein family includes eight members in humans: SOCS-1 through SOCS-7 and cytokine-inducible Src-homology 2 (SH2) containing protein (CIS). All members of the family have similar structure: variable length (50-380aa) and aa sequence N-terminal region, SH2 domain in the center, and homologous C-terminal 40aa long sequence that was named SOCS box. Studies have demonstrated that animals lacking normal SOCS function exhibit high cytokine activity that leads to a variety of inflammatory and autoimmune diseases and even cancer. SOCS-6 is one of the least studied members of the SOCS family. It is 535aa long and has 56% sequence identity with SOCS-7 in region (1-2, 7). Recent studies revealed that SOCS-6 does not interact with Janus kinase family proteins. However, it was found that SOCS-6 binds to KIT membrane region and negatively regulates receptor tyrosine kinases. In addition, studies revealed that SOCS-6 binds to insulin receptor substrate-2, and -4 (IRS-2, and 4) and inhibits downstream signaling.
Suitable for use in ELISA and Western Blot. Other applications not tested.
Western Blot: 0.5-2ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.