Accumulating evidence demonstrates that cytokine receptor signaling is negatively regulated by a family of Src homology 2 domain-containing adaptor molecules termed SOCS (Suppressor of Cytokine Signaling). To date, eight members of SOCS family have been recognized, they are SOCS-1, 2, 3, 4, 5, 6, 7 and CIS. Structurally the SOCS proteins are composed of an N-terminal region of variable length and aa composition, a central SH2 domain, and a previously unrecognized C- terminal motif that was called the SOCS box. The SOCS proteins appear to form part of a classical negative feed back loop that regulates cytokine signal transduction via a STAT-induced transcriptional mechanism. Transcription of each of the SOCS genes occurs rapidly in vitro and in vivo in response to cytokines, and once produced, the various members of the SOCS family appear to inhibit signaling in different ways. Within the SOCS subfamily, SOCS4, SOCS-5, SOCS6, SOCS7 remain poorly understood. On the basis of structural considerations, it would be expected that SOCS4-SOCS-7, like SOCS1, SOCS2, SOCS3 and CIS, acted to negatively regulate signal transduction. SOCS5 might be involved in cellular apoptosis, it suppressed TNF-a induced cell dead at a low dose, no effect at a high dose.
Suitable for use in ELISA and Western Blot. Other applications not tested.
Western Blot: 0.5-2ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.