Suppressors of cytokine signaling (SOCS) protein family includes eight members in humans: SOCS-1 through SOCS-7 and cytokine-inducible Src-homology 2 (SH2) containing protein (CIS). All members of the family have similar structure: variable length (50-380aa) and aa sequence N-terminal region, SH2 domain in the center, and homologous aa long sequence at the C-terminus that was named SOCS box. Studies have demonstrated that animals lacking normal SOCS function exhibit high cytokine activity that leads to a variety of inflammatory and autoimmune diseases and even cancer. SOCS-2 is 198aa long with short N-terminus and shares sequence similarity with CIS. SOCS-2 mRNA normally presents at very low levels in different tissues; however, its level is rapidly increased upon stimulation with IL-1, IL-3, IL-4, IL-6, LIF, GM-CSF, G-CSF, EPO, IFNg, prolactin, and GH. Similar to CIS, SOCS-2 does not bind to Janus- activated kinases; however, it can associate with GH, IGF-1, prolactin and leptin receptors. For example, SOCS-2 binds to Y1077 motif of leptin receptor and thus acts as a negative regulator of STAT5.
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Western Blot: 1:500-1:1000
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.