Suppressors of cytokine signaling (SOCS) protein family includes eight members in humans: SOCS-1 through SOCS-7 and cytokine-inducible Src-homology 2 (SH2) containing protein (CIS). All members of the family have similar structure: variable length (50-380aa) and aa sequence N-terminal region, SH2 domain in the center, and homologous C-terminal 40aa long sequence that was named SOCS box. Studies have demonstrated that animals lacking normal SOCS function exhibit high cytokine activity that leads to a variety of inflammatory and autoimmune diseases and even cancer. SOCS-1 is 211aa long and shares sequence similarity with SOCS-3. Its expression in many tissues is induced by elevated levels of IL-2, IL-3, IL- 6, IL-13, LIF, GM-CSF, IFNg, GH, and prolactin. SOCS-1 targets Janus activated kinases 1-3 (JAK1-3) and tyrosine kinases 2 (TYK2). These events negatively regulate family of signal transducer and activator of transcription (STAT) proteins. For example, SOCS-1 binds to Y1007 residue of JAK2 and blocks its phosphorylation activity that does not allow activation of STAT3.
Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution:
ELISA: 0.1-1ug/ml
Western Blot: 1-2ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.