SNAP 25 (Synaptosomal-associated Protein 25, Synaptosomal Associated Protein 25, Synaptosomal-associated 25kD Protein, Synaptosomal-associated Protein 25kDa, SNAP25, SNAP-25, bA416N4.2, dJ1068F16.2, FLJ23079, HGNC:11132, Resistance to Inhibitors of Cholin

SNAP 25 (synaptosome-associated protein of 25kD) is a SNAP (soluble N-ethylmaleimide-sensitive fusion (NSF) attachment protein) receptor (SNARE-related protein) which is tightly associated with the plasma membrane by means of the palmitoylation of a cluster of cysteine residues with its structure. SNAP 25 is specifically cleaved close to the carboxyl terminal by the botulinum neurotoxins (BoNT) A and E which are known to inhibit neurotransmitter release. Similar inhibitory effects of BoNT/A and E have been recorded for non-neuronal secretory cells, such as adrenal medulla, This leads to the identification of SNAP 25 in SNARE-like complexes precipitated from chromaffin cells.\n\nApplications:\nSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 1:500-1:5000. Detects a band at ~26-27kD.\nImmunohistochemistry (Paraffin): 1:2000-1:5000. No pretreatment or antigen retrieval using heat treatment is required.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

S1014-73C

Size

100ug

Applications

ELISA, IHC, WB

Hosts

Mouse

Reactivities

Hum, Rat, Prc

Form

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG1

References

1. Honer, W.G., et al., Hippocampal synaptic pathology in patients with temporal lobe epilepsy. Acta Neuropathol (Berl) 87: 202-210 (1994). 2. Honer, W.G., et al., Human synaptic proteins with a heterogeneous distribution in cerebellum and visual cortex. Brain Res. 609: 9-20 (1993). 3. Honer, W.G., et al., Regional synaptic pathology in Alzheimer's disease. Neurobiol. Aging 13: 375-382 (1992). 4. Honer, W.G., et al., Characterization of a synaptic antigen of interest in neuropsychiatric illness. Biol Psychiatry 31: 147-158 (1992).

Additional Info

Recognizes human SNAP 25. Species Crossreactivity: rat and porcine.