Smoothelin is one of the smooth muscle cell (SMC) specific proteins such as calponin, alpha smooth muscle cell actin, SM22alpha and smooth muscle myosin heavy chain isoforms. Smoothelin is exclusively expressed in fully differentiated (contractile) SMCs. It colocalizes with actin stress fibers, this distribution indicates a correlation between smoothelin expression and smooth muscle tissue contractility. Two major isoforms of smoothelin have been reported, a 59kD smoothelin-A in visceral smooth muscle cells and a 110kD smoothelin-B in vascular smooth muscle cells. Both gene products are encoded by a single copy gene which is located on chromosome 22 and regulated by a dual promoter system in a tissue specific manner. The variation in the smoothelin proteins is achieved by alternative splicing in the calponin homology domain. Antibodies to smoothelin are powerful tools in evaluation of smooth muscle cell differentiation as occurs in atherosclerotic lesions.
Suitable for use in Immunofluorescence, Flow Cytometry, Western Blot and Immunohistochemistry. Other applications not tested.
Flow Cytometry: 1:50
Western Blot: 1:50
Immunohistochemistry (Frozen): 1:50
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.