Home  >  Products  >  SMC3 (Structural Maintenance of Chromosomes Protein 3, SMC Protein 3, SMC-3, Basement Membrane-associated Chondroitin Proteoglycan, Bamacan, Chondroitin Sulfate Proteoglycan 6, Chromosome-associated Polypeptide, hCAP, BAM, BMH, CSPG6, SMC3L1)
SMC3 (Structural Maintenance of Chromosomes Protein 3, SMC Protein 3, SMC-3, Basement Membrane-associated Chondroitin Proteoglycan, Bamacan, Chondroitin Sulfate Proteoglycan 6, Chromosome-associated Polypeptide, hCAP, BAM, BMH, CSPG6, SMC3L1)

SMC3 (Structural Maintenance of Chromosomes Protein 3, SMC Protein 3, SMC-3, Basement Membrane-associated Chondroitin Proteoglycan, Bamacan, Chondroitin Sulfate Proteoglycan 6, Chromosome-associated Polypeptide, hCAP, BAM, BMH, CSPG6, SMC3L1)

Cat no: S7982-03F

Supplier: United States Biological
Star_fadedStar_fadedStar_fadedStar_fadedStar_faded
0 reviews | Write a Review Pencil
Read More on Supplier Website
The cohesin complex consists of a heterodimer between SMC1 (SMC1A or B) and SMC3, bound by additional RAD21 and STAG proteins (STAG1, 2, or 3) (1,2). These proteins form a ring-like structure that mediates the cohesion of two sister chromatids after DNA replication in S phase (1,2). RAD21 and STAG2 are phosphorylated by Polo-like kinase (PLK) during prophase, which leads to the dissociation of cohesin complexes from the chromosome arms; however, cohesin remains bound to centromeres until anaphase (3,4). RAD21 is cleaved by separin/ESPL1 in anaphase, which leads to dissociation of the remaining cohesin from centromeres, enabling sister chromatids to segregate during mitosis (5). RAD21 is also cleaved by caspase-3 and caspase-7 during apoptosis, resulting in a 64kD carboxy-terminal cleavage product that translocates to the cytoplasm and may help to trigger apoptosis (6,7). In addition to mediating cohesion of sister chromatids, the cohesin complex plays important roles in gene regulation and DNA repair, as SMC1 and SMC3 are both phosphorylated by ATM and ATR kinases upon DNA damage (1,2). Applications: Suitable for use in Immunofluorescence, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Immunofluorescence (IF-IC): 1:200 Western Blot: 1:2000 Immunoprecipitation: 1:200 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: S7982-03F
Reactivities: Human, Mouse, Rat, Bovine, Chicken/Bird, Non-Human Primate, Xenopus/Amphibian, Zebrafish/Fish
Hosts: Rabbit
Applications: Immunofluorescence, Immunoprecipitation, Western Blot
Size: 100ul
Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, <0.02% sodium azide, 50% glycerol.
P type: Mab
Isotype: IgG
Purity: Supernatant
References: 1.Peters, J.M. et al. (2008) Genes Dev 22, 3089-114. 2.Barbero, J.L. (2009) Cell Mol Life Sci 66, 2025-35. 3.Hoque, M.T. and Ishikawa, F. (2001) J Biol Chem 276, 5059-67. 4.Hauf, S. et al. (2005) PLoS Biol 3, e69. 5.Hauf, S. et al. (2001) Science 293, 1320-3. 6.Pati, D. et al. (2002) Mol Cell Biol 22, 8267-77. 7.Chen, F. et al. (2002) J Biol Chem 277, 16775-81.
Additional info: Recognizes endogenous levels of total human SMC3. Species Crossreactivity: Bovine, chicken, monkey, mouse, rat, xenopus and zebrafish.

Get Quote

  • Best Price Guaranteed
  • Quick Response Time
  • Exclusive Promotions
Enquiry_down_arrow
United States Biological
Get a Quote Direct from
United States Biological

By submitting this form you agree to your details being passed to United States Biological for the purpose of generating the best quote*
See Full Product Listings >
Button_on Button_off_biosave Button_off_biosave Button_off_biosave Button_off_biosave Button_off_biosave Button_off_biosave