Home  >  Products  >  Smad3, phosphorylated (Ser423/425) (SMAD Family Member 3, DKFZp586N0721, DKFZp686J10186, hMAD-3, hSMAD3, HSPC193, HsT17436, JV15-2, Mad3, MADH3, MGC60396, Mothers Against Decapentaplegic Homolog 3, Mothers Against DPP Homolog 3)
Smad3, phosphorylated (Ser423/425) (SMAD Family Member 3, DKFZp586N0721, DKFZp686J10186, hMAD-3, hSMAD3, HSPC193, HsT17436, JV15-2, Mad3, MADH3, MGC60396, Mothers Against Decapentaplegic Homolog 3, Mothers Against DPP Homolog 3)

Smad3, phosphorylated (Ser423/425) (SMAD Family Member 3, DKFZp586N0721, DKFZp686J10186, hMAD-3, hSMAD3, HSPC193, HsT17436, JV15-2, Mad3, MADH3, MGC60396, Mothers Against Decapentaplegic Homolog 3, Mothers Against DPP Homolog 3)

Cat no: S1014-53P2

Supplier: United States Biological
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Members of the Smad family of signal transduction molecules are components of a critical intracellular pathway that transmits TGF-beta signals from the cell surface into the nucleus. Three distinct classes of Smads have been defined: the receptor-regulated Smads (R-Smads), which include Smad1, 2, 3, 5 and 8, the common-mediator Smad (co-Smad), Smad4, and the antagonistic or inhibitory Smads (I-Smads), Smad6 and 7. Once in the nucleus, Smads can target a variety of DNA binding proteins to regulate transcriptional responses. Following stimulation by TGF-beta, Smad2 and Smad3 become phosphorylated at their carboxyl termini (Ser465 and 467 on Smad2; Ser423 and 425 on Smad3) by TGF-beta Receptor I. Phosphorylated Smad 2/3 can complex with Smad4, translocate to the nucleus and regulate gene expression. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: S1014-53P2
Reactivities: Human, Mouse
Hosts: Rabbit
Applications: Western Blot
Size: 100ul
Form: Supplied as a liquid, 0.05% sodium azide.
P type: Pab
Isotype: IgG
Purity: Serum.
References: 1. Mohan, R., et al. (2002) J Biol Chem, 277:2065-2072
Additional info: Recognizes phosphorylated Smad3 (Ser423 and Ser425). Species Crossreactivity: mouse and mink. Species sequence homology: human, rat.

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