Home  >  Products  >  Smad2 (SMAD Family Member 2, SMAD 2, SMAD-2, hMAD-2, hSMAD2, JV18-1, JV18, MAD-related Protein 2, MADR2, Mothers Against Decapentaplegic Homolog 2, Mothers Against DPP Homolog 2, MAD Homolog 2, MADH2)
Smad2 (SMAD Family Member 2, SMAD 2, SMAD-2, hMAD-2, hSMAD2, JV18-1, JV18, MAD-related Protein 2, MADR2, Mothers Against Decapentaplegic Homolog 2, Mothers Against DPP Homolog 2, MAD Homolog 2, MADH2)

Smad2 (SMAD Family Member 2, SMAD 2, SMAD-2, hMAD-2, hSMAD2, JV18-1, JV18, MAD-related Protein 2, MADR2, Mothers Against Decapentaplegic Homolog 2, Mothers Against DPP Homolog 2, MAD Homolog 2, MADH2)

Cat no: S1014-52L

Supplier: United States Biological
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Members of the Smad family of signal transduction molecules are components of a critical intracellular pathway that transmit TGF-B signals from the cell surface into the nucleus. Three distinct classes of Smads have been defined: the receptor-regulated Smads (R-Smads), which include Smad1, 2, 3, 5 and 8; the common-mediator Smad (co-Smad), Smad4; and the antagonistic or inhibitory Smads (I-Smads), Smad6 and 7 (1-5). Activated type I receptors associate with specific R-Smads and phosphorylate them on a conserved carboxy-terminal SSXS motif. The phosphorylated R-Smad dissociates from the receptor and forms a heteromeric complex with the co-Smad (Smad4), allowing translocation of the complex to the nucleus. Once in the nucleus, Smads can target a variety of DNA binding proteins to regulate transcriptional responses (6-8). Applications: Suitable for use in Immunofluorescence, Flow Cytometry, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Immunofluorescence (IF-IC): 1:50 Flow Cytometry: 1:200 Western Blot: 1:1000 Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4 degrees C with gentle shaking, overnight. Immunoprecipitation: 1:50 Chromatin Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: S1014-52L
Reactivities: Human, Mouse, Rat, Non-Human Primate
Hosts: Rabbit
Applications: Flow Cytometry, Immunofluorescence, Immunoprecipitation, Western Blot
Size: 100ul
Form: Supplied a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, < 0.02% sodium azide, 50% glycerol.
P type: Mab
Isotype: IgG
Purity: Supernatant
Alternative names: MGC22139, MGC34440
References: 1) Heldin, C.H. et al. (1997) Nature 390, 465-471. (2) Attisano, L. and Wrana, J.L. (1998) Curr. Opin. Cell Biol. 10, 188-194. (3) Derynck, R. et al. (1998) Cell 95, 737-740. (4) Massague, J. (1998) Annu. Rev. Biochem. 67, 753-791. (5) Whitman, M. (1998) Genes Dev. 12, 2445-2462. (6) Wu, G. et al. (2000) Science 287, 92-97. (7) Attisano, L. and Wrana, J.L. (2002) Science 296, 1646-1647.
Additional info: Recognizes endogenous levels of total Smad2. Species Crossreactivity: human, rat, monkey.

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