SLP 76, phosphorylated (Tyr145) (SH2-domain Leukocyte Protein)

The activation of protein tyrosine kinases is a critical event in T cell antigen receptor (TCR)-mediated signaling. One substrate of the TCR-activated protein tyrosine kinase pathway is a 76-kD protein (SLP-76) that associates with the adaptor protein Grb2. Northern analysis demonstrates that SLP76 mRNA is expressed solely in peripheral blood leukocytes, thymus, and spleen; and in human T cell, B cell and monocytic cell lines. In vitro translation of SLP76 cDNA gives rise to a single product of 76kD that associates with a GST/Grb2 fusion protein, demonstrating a direct association between these two molecules. It has been reported that this novel protein is likely to play an important role in TCR-mediated intracellular signal transduction (1). It has been shown that SDF-1alpha stimulation of prolonged ERK activation in Jurkat T cells requires both the ZAP-70 tyrosine kinase and the Src homology 2 domain-containing leukocyte protein of SLP-76 scaffold protein. This pathway involves ZAP-70-dependent tyrosine phosphorylation of SLP-76 at one or more of its tyrosines, 113, 128, and 145 (2). Applications: Suitable for use inFlow Cytometry, Western Blot, Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:100,000-1:200,000 Immunocytochemistry: 1:100 Flow Cytometry: 1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Manufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.