Solute Carrier Family 5 Member 1 (Slc5a1/SGLT1) is a member of the sodium dependent glucose transporters also known as co transporters or symporters. It is an integral membrane protein involved in the active transport of glucose and galactose across the membrane of cells lining the gi tract and kidney. Transport is driven by the sodium/potassium ATPase.
Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilutions:
Western Blot: 0.5-4ug/ml. Detects a band at ~73kD from human samples and in a lesser extant from mouse and rat samples. A 50kD cleavage fragment can also be detected in Jurkat cell lysate.
Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml. Validated for use on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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