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SIX2 (Sine Oculis Homeobox Homolog 2)

Cat no: S1014-08D

Supplier: United States Biological
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SIX2 is a member of the vertebrate gene family that is homologous to the Drosophila 'sine oculis' homeobox protein. It is a transcription factor that, like other members of this family, may be involved in limb or eye development (1). SIX2 is expressed in a subset of metanephric mesenchyme, where its expression is maintained throughout kidney development; no expression is detected in adult mouse kidneys (2). Moreover, it is strongly expressed in skeletal muscle (3). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000-10,000 Optimal dilutions to be determined by the researcher. Positive Control: Western Blot: Human skeletal muscle, mouse skeletal muscle, rat skeletal muscle, and L6 lysates Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Manufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
Catalogue number: S1014-08D
Reactivities: Human, Mouse, Rat
Hosts: Rabbit
Applications: Western Blot
Size: 100ul
Form: Supplied as a liquid in 50mM tris-glycine, pH7.4, 0.15M NaCl, 40% glycerol, 0.01% sodium azide, 0.05% BSA.
P type: Mab
Purity: Supernatant
References: 1. Entrez Gene: gene-centered information at NCBI. Nucleic Acids Res. 2005 Jan 1;33:D54-8. 2. Kobayashi A et al. Cell Stem Cell. 2008 Aug 7;3(2):169-81. 3. The UniProt Consortium The Universal Protein Resource (UniProt) in 2010 Nucleic Acids Res. 38:D142-D148 (2010).
Additional info: Recognizes human SIX2 at ~32kD. Species Crossreactivity: mouse, rat.

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