Human CD172a, also known as signal-regulatory protein alpha, a receptor-type transmembrane glycoprotein expressed on cells of myeloid origin, including granulocytes, dendritic cells (DCs),
macrophages, mast cells and haematopoietic stem cells. CD172a acts as a substrate for several activated tyrosine kinases, including EGFR, PDGFR, src and insulin receptor and is involved in the negative regulation of receptor tyrosine kinase-coupled signaling pathways. Ligand binding of CD172a to integrin-associated protein CD47, results in tyrosine kinase phosphorylation of immunoreceptor tyrosine-based inhibitory motifs (ITIMs) within the cytoplasmic region of CD172a, mediating the recruitment and activation of the tyrosine phosphatases SHP-1 and SHP-2. These then act as regulators of cellular function, through dephosphorylation of specific substrates. Ligation of CD172a with CD47 has been demonstrated in several regulatory processes, including the inhibition of host cell phagocytosis by macrophages and the bi-directional activation of T cells and DCs.
Applications:
Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Flow Cytometry: 1:25-1:100. Use 10ul to label 1x10e6 cells in 100ul.
Immunohistochemistry (Frozen): 1:100
Optimal dilutions to be determined by the researcher.
Hybridoma:
Spleen cells from immunized Balb/c mice were fused with cells of the X63-Ag8.653 myeloma cell line
Recommended Positive Control (Ihc):
Human Tonsil
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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