SIK2 is a cytoplasmic 125kD member of the CAMK Ser/Thr protein kinase family, AMPK subfamily of enzymes.It is expressed in preadipocytes/adipocytes, with levels strongly induced during early differentiation. SNF1LK2 regulates the early phase of insulin signaling by phosphorylating IRS-1 on Ser794.Human SNF1LK2 is 926aa in length and contains a protein kinase domain (aa20-271) with an ATP binding site (Lys49) and a catalytic loop (aa140-149), followed by a phosphorylation activation site at Thr175, a UBA (ubiquitin-associated) domain (aa295-335) and another PKA-dependent phosphorylation site at Ser587.
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Western Blot: 1-3ug/ml
Immunohistochemistry (Formalin fixed paraffin embedded): 10ug/ml
IOptimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.