Siglec 7 is a type I transmembrane glycoprotein and member of the Siglec (sialic acid binding Ig-like lectin) family, originally identified as an inhibitory NK cell receptor (NKR) and negative regulator of NK activation, attributed to ITIM recruitment of SHP-1 phosphatase. Siglec 7 is expressed predominantly by natural killer cells (NK) and to a lesser extent by monocytes and granulocytes and, like Siglec-5 (CD170), has been shown to bind to sialylated ligands of targets through recognition of sialic acid in both the alpha-2,3- and alpha-2,6- glycosidic linkage.
Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.
Flow Cytometry: 1:100-1:200; 10ul labels 1x10e6 cells in 100ul.
Immunohistochemistry (Frozen): 1:100
Optimal dilutions to be determined by the researcher.
IHC Positive Control Tissue:
Recommended Negative Control:
I1904-78R: IgG1 Murine Negative Control
X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.