Suitable for use in Flow Cytometry and Immunohistochemistry (frozen). Other applications not tested.
Flow Cytometry: 1:100-1:200; 10ul labels 10e6 cells in 100ul
Immunohistochemistry (frozen): 1:100
Optimal dilutions to be determined by the researcher.
X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.