Siah-1 is a member of the seven in asentia homolog (SIAH) family. It is a E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins, including many proteins involved in transcription regulation, a cell surface receptor (DCC), cytoplasmic signal transduction molecules, an antiapoptotic protein (BAG1), a microtubule motor protein (KIF22), a protein involved in synaptic vesicle function in neurons (SYP) and a structural protein. Consequetly Siah-1 is involved in many cellular processes such as apoptosis, tumor suppression, cell cycle, axon gyidance, transcription regulation, spermatogenesis, and TNF-alpha signaling. It is predominantly a cytoplasmic protein but is also partialy a nuclear protein. It is widely expressed at low levels and is down-regulated in advanced hepatocellular carcinomas.
Applications:
Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Immunofluorescence: 10ug/ml
Immunohistochemistry: 3ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile 40-50% glycerol, ddH2O. Reconstituted product is stable for 12 months at -20 degrees C. Aliquot and store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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