Stabilin-interacting chitinase like protein (SI-CLP), a novel member of the Glyco_18-domain containing protein family. SI-CLP is a secretory protein which is up-regulated in response to interleukin-4 and dexamethasone in alternatively activated macrophages. SI-CLP is also expressed by T- and B-lymphocytes, and by cells of monocytic and epithelial origin. SI-CLP is the ligand for stabilin-1, an intracellular sorting receptor which sorts SI-CLP into late endosomes and secretory lysosomes in activated macrophages.
Suitable for use in Flow Cytometry, Immunofluorescence, and Western Blot. Other applications not tested.
Flow Cytometry: 10ul labels 10e6 cells in 100ul
Membrane permeabilisation is required.
Western Blot: Detects a band at ~33 and 39kD in U937 cell lysates.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.