Shootin1

Cat no: S1013-24

Supplier: United States Biological

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The differentiation process of neurons can be divided into five stages, each stage characterized by morphological changes observed in the developing cells. In stage 1, the cells extend lamellipodia and in stage 2 their lamellipodia develop into immature neurites. In stage 3 one neurite elongates rapidly to form an axon and in stage 4 the remaining immature neuritis elongate to form dendrites. In stage 5 synaptic contacts are formed and a neuronal network is established (1). Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunoprecipitation: 1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Catalogue number:	S1013-24
Reactivities:	Human, Mouse, Rat
Hosts:	Rabbit
Applications:	Immunoprecipitation, Western Blot
Size:	100ul
Form:	Supplied as a liquid in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100ug/ml BSA and 50% glycerol.
P type:	Pab
Isotype:	IgG
Purity:	Purified by peptide affinity chromatography.
References:	(1) Yoshimura, T. et al. (2006) J Neurosci 26, 10626
Additional info:	Detects endogenous levels of total human shootin1 protein. Species crossreactivity: mouse and rat.

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Shootin1

Cat no: S1013-24

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