Shc1 is an adapter protein in the Ras-MAPkinase pathway that is involved in the regulation of cell growth and differentiation. The protein contains both PTB and SH2 domains and becomes phosphorylated on tyrosine in response to many different extracellular stimuli. Human Shc1 adaptor protein is encoded by one gene but has three isoforms: p66SHC1, p52SHC1 and p46SHC1. Both p52SHC1 and p46SHC1 are associated with MAPK signaling and are highly conserved throughout evolution; p66SHC1 signaling is associated with apoptosis and is only seen in vertebrates. The SHC1 gene contains one known coding variation, which corresponds to Met225Val in the p46 isoform, Met300Val in the p52 isoform and to Met410Val in the p66 isoform. Recent studies confirms Shc1 an important candidate gene for longevity in humans. The protein is widely expressed including the neural stem cells.
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Western Blot: 1:500-1:1000
Immunohistochemistry (paraffin): 1:50-1:100
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.