SH3BP2 binds differentially to the SH3 domains of certain proteins of signal transduction pathways. It also binds to phosphatidylinositols, thereby linking the hemopoietic tyrosine kinase, fes, to the cytoplasmic membrane in a phosphorylation dependent mechanism.
Phosphorylation of SH3BP2 occurs on Ser 427 for activation. SH3BP2 mediates interactions of huntingtin and MLK2 (mixed lineage kinase).
Suitable for use in ELISA and Western Blot. Other applications not tested.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.