SH3PXD2B is a novel adaptor protein; also known as SH3 and PX domain-containing protein 2B, (Courtneidge, 2005). It is closely related to Tks5 (gene symbol SH3PXD2A) and like Tks5, appears to play a role in the formation and function of podosome/invadopodia based on studies of Src-transformed fibroblasts (Buschman, 2009). Additionally, SH3PXD2B may be involved in the recruitment of MT1-MMP and ECM degradation.
SH3PXD2B is broadly expressed and localizes in the cytoplasm of the podosome.
Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.
Western Blot: 3ug/ml
Optimal dilutions to be determined by the researcher.
NIH3T3 cell lysate
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.