SH3PXD2B is a novel adaptor protein; also known as SH3 and PX domain-containing protein 2B, (Courtneidge, 2005). It is closely related to Tks5 (gene symbol SH3PXD2A) and like Tks5, appears to play a role in the formation and function of podosome/invadopodia based on studies of Src-transformed fibroblasts (Buschman, 2009). Additionally, SH3PXD2B may be involved in the recruitment of MT1-MMP and ECM degradation.
SH3PXD2B is broadly expressed and localizes in the cytoplasm of the podosome.
Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested.
Western Blot: 3ug/ml. 50ug of NIH3T3 IP cell lysate was resolved via SDS-PAGE, transferred to PVDF and probed with 3ug/ml S1013-33A. Proteins were visualized using an IgG (HRP) anti-rabbit that does not recognize denatured IgG. Detection using a chemiluminescence system.
Immunoprecipitation: 10ug immunoprecipitates 500ug of NIH3T3 whole cell lysate
Optimal dilutions to be determined by the researcher.
NIH3T3 cell lysate
Recommended Secondary Antibodies:
I1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb
I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb
I1904-46Q: IgG, H&L (HRP) Pab Gt xRb
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.