Home  >  Products  >  SH3 and PX domain-containing protein 2B (SH3PXD2B, Factor for adipocyte differentiation 49, SH3 and PX domains 2B, Factor for adipocyte differentiation 49, fad49, Fad49, G431001E03Rik)
SH3 and PX domain-containing protein 2B (SH3PXD2B, Factor for adipocyte differentiation 49, SH3 and PX domains 2B, Factor for adipocyte differentiation 49, fad49, Fad49, G431001E03Rik)

SH3 and PX domain-containing protein 2B (SH3PXD2B, Factor for adipocyte differentiation 49, SH3 and PX domains 2B, Factor for adipocyte differentiation 49, fad49, Fad49, G431001E03Rik)

Cat no: S1013-33A

Supplier: United States Biological
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SH3PXD2B is a novel adaptor protein; also known as SH3 and PX domain-containing protein 2B, (Courtneidge, 2005). It is closely related to Tks5 (gene symbol SH3PXD2A) and like Tks5, appears to play a role in the formation and function of podosome/invadopodia based on studies of Src-transformed fibroblasts (Buschman, 2009). Additionally, SH3PXD2B may be involved in the recruitment of MT1-MMP and ECM degradation. SH3PXD2B is broadly expressed and localizes in the cytoplasm of the podosome. Applications: Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 3ug/ml. 50ug of NIH3T3 IP cell lysate was resolved via SDS-PAGE, transferred to PVDF and probed with 3ug/ml S1013-33A. Proteins were visualized using an IgG (HRP) anti-rabbit that does not recognize denatured IgG. Detection using a chemiluminescence system. Immunoprecipitation: 10ug immunoprecipitates 500ug of NIH3T3 whole cell lysate Immunocytochemistry: 1:3000 Optimal dilutions to be determined by the researcher. Positive Control: NIH3T3 cell lysate Recommended Secondary Antibodies: I1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb I1904-46Q: IgG, H&L (HRP) Pab Gt xRb Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: S1013-33A
Reactivities: Human, Mouse
Hosts: Rabbit
Applications: Immunocytochemistry, Immunoprecipitation, Western Blot
Size: 100ug
Form: Supplied as a liquid in Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
P type: Pab
Isotype: IgG
Purity: Purified by Protein A affinity chromatography.
References: 1. Buschman, M.D., et al., (2009). Mol. Cell Biol. 20(5):1302-1311. 2. Courtneidge, S.A., et al., (2005). Cold Spring Harb Symp Quant Biol. 70:167-171.
Additional info: Recognizes mouse SH3PXD2B at ~130kD. Species Crossreactivity: human. Species Sequence Homology: rat (96%), equine (88%), canine (87%) and bovine (87%).

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