RIBP (Rlk/Itk-binding protein; also SH2D2A, LAD/Lck-associated adapter protein, MEKK2-binding protein and TSAd) is a 45-47kD cytoplasmic adaptor protein. It is found in B cells, thymocytes, NK cells and T cells, and appears to be necessary for TCR-initiated proliferation, plus IL-2 and IFN-gamma secretion. This is likely to be a function of its association with MEKK2 near the T cell receptor interaction site. Mouse RIBP is 374aa in length and contains one SH2 domain (aa116-207), plus a Pro-rich region (aa267-308) that contains an SH3-binding motif (aa267-273). Phosphorylation may occur on Ser237, Ser316 and Tyr325. There is one alternative splice form termed LAD that shows a deletion of aa254-261. Over aa299-374, mouse RIBP shares 79% and 63% aa identity with rat and the presumed human RIBP ortholog (termed TSAd/SH2D2A), respectively.
Applications:
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilutions:
Western Blot: 1ug/ml Detects a band at ~45kD.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS, 0.02% sodium azide. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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