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SFN, NT (14-3-3 Protein sigma, Epithelial Cell Marker Protein 1, Stratifin, HME1)

Cat no: S7973-69H


Supplier: United States Biological
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Members of the 14-3-3 family of proteins are highly conserved proteins, localized in neurons, and are axonally transported to the nerve terminals. They are also present, at lower levels, in various other eukaryotic tissues. 14-3-3 proteins appear to play important roles in a variety of signal transduction pathways, including those involved in cell cycle regulation and cell survival. As 14-3-3 proteins bind to specific phosphoserine-containing sequences they are likely to have an important role in signaling pathways mediated by serine/threonine protein kinases. 14-3-3 sigma may be required for Raf-1 kinase activity and phosphorylation, among many other functions. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry (Formalin fixed paraffin embedded): 1:250 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: S7973-69H
Reactivities: Sheep
Hosts: Rabbit
Applications: Immunohistochemistry, Western Blot
Size: 50ul
Form: Supplied as a liquid , 0.09% sodium azide.
P type: Pab
Isotype: IgG
Purity: Serum
Additional info: Recognizes 14-3-3 sigma in all mammals. Species sequence homology: Bovine, chicken, human, mammal, mouse, rabbit, rat.

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