Scavenger Receptor BI (Scavenger Receptor Class B Member 1, Scavenger Receptor Class B Type 1, Scavenging Receptor SR-BI, SCARB1, SRB1, SRBI, SR-BI, CD36 and LIMPII Analogous 1, CD36 Antigen-like 1, CD36L1, CLA1, CLA-1, Collagen Type I Receptor, MGC138242

Monoclonal antibody 3D12 reacts with rat class B scavenger receptor type I (SR-BI). Scavenger receptors have been studied primarily for their ability to bind and internalize modified lipoproteins. They have been found in the development of atherosclerosis and other macrophage-associated functions. Scavenger receptors also function as pattern recognition receptors for a wide variety of pathogens. This finding indicates a potential role in host defense. SR-BI belongs together with CD36 to the class B scavenger receptor family. SR-BI is a multiligand membrane protein existing in various organs such as the liver and various cell types such as endothelial cells, macrophages, brain cells, Leydig cells and Sertoli cells. SR-BI has been found as a receptor for phospholipids, free and (lipo)protein-bound ApoE, lipid-bound ApoA-I, HDL, hypochlorite-modified LDL and more. In liver, the PDZK-1 (and possible other PDZ domains) of SR-BI has been found to be essential for cell surface expression and, hence, reverse cholesterol transport. In the brain, the presence of SR-BI seems to be involved in the uptake of oxidatively modified lipoproteins and beta-amyloid protein complexed with ApoE, suggesting SR-BI to be an important tool for studies on neurodegenerative disorders. In the testis, SR-BI is expressed in two somatic cell types: Leydig cells and Sertoli cells. SR-BI functions at least partly as a phosphatidyl serine receptor (PSR), enabling Sertoli cells to recognize and phagocytose apoptotic spermatogenic cells at all stages of differentiation. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry, and Neutralization of the biological activity of SR-BI. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:50 Immunohistochemistry (frozen): 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.