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PTEN Tumor Suppressor Protein (PTEN, MMAC1, Phosphatase and Tensin Homolog, TEP1)

Cat no: P9182-05E

Supplier: United States Biological
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Applications: Suitable for use in ELISA, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation and Western Blot. Other applications have not been tested. Recommended Dilutions: Immunoprecipitation: 5microg Western Blot: 1-5ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: P9182-05E
Reactivities: Human, Mouse, Rat
Hosts: Mouse
Applications: ELISA, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Size: 100ug
Form: Supplied as a liquid in PBS.
P type: Mab
Isotype: IgG
Purity: Purified by Protein A-affinity chromatography.
References: 1. Immunohistochemical evidence of loss of PTEN expression in primary ductal adenocarcinomas of the breast: A. Perren, et al.; Am. J. Pathol. 155, 1253 (1999) 2. PTEN suppresses breast cancer cell growth by phosphatase activity- dependent G1 arrest followed by cell death: L.P. Weng, et al.; Cancer Res. 59, 5808 (1999) 3. Altered PTEN expression as a diagnostic marker for the earliest endometrial precancers: G.L. Mutter, et al.; J. Natl. Cancer Inst. 92, 924 (2000) 4. Changes in endometrial PTEN expression throughout the human menstrual cycle: G.L. Mutter, et al.; J. Clin. Endocrinol. Metab. 85, 2334 (2000) 5. Molecular identification of latent precancers in histologically normal endometrium: G.L. Mutter, et al.; Cancer Res. 61, 4311 (2001)
Additional info: Recognizes human PTEN (epitope aa 304-403). Detects a band of ~60kD by Western blot. Species crossreactivity: mouse and rat.

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