6-keto-Prostaglandin F1a (6-k-PGF1a) is a stable hydrolyzed product of unstable PGI2 (prostacyclin). PGI2 is derived from PGH2 which in turn is synthesized from the cyclooxygenase pathway. Through this pathway, it is the major product produced by the endothelial cells, although it is also synthesized in other cell types such as macrophages. Prostacyclin inhibits platelet aggregation and induces vasodilation. Quantitation of prostacyclin production can be made by determining the level of 6-k-PGF1a.
Kit Components:
P9053-31A: EIA Buffer, 1x30ml
P9053-31B: Wash Buffer (10X), 1x20ml
P9053-31C: Substrate (TMB), 1x20ml
P9053-31D: Extraction Buffer (5X), 1x30ml
P9053-31E: Conjugate (HRP), 1x150ul
P9053-31F: Standard (1ug/ml), 1x100ul
P9053-31G: Microtiter plate, 1x96 wells
Storage and Stability:
Store all components at 4 degrees C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
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