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Phosphatase Assay Kit, Alkaline/Acid RRA-pS-VA, BioAssay(TM)

Phosphatase Assay Kit, Alkaline/Acid RRA-pS-VA, BioAssay(TM)

Cat no: P4071-60

Supplier: United States Biological
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Phosphatase Assay Kit, Alkaline/Acid RRA-pS-VA, BioAssay(TM) is suitable for detection and quantitation of acid and alkaline phosphatase activity from cell extracts. Capacity: Quantity: 100 assays per kit. Quality Control: The components of this kit have matched to optimize sensitivity and range of detection. Phosphate released is determined by comparing absorbance to standard curve of step 2. Using this kit, 0.5U of calf intestine alkaline phosphatase released 66.9pmol phosphate per minute using the phosphopeptide (RRApSVA). Note: Do not perform the Malachite Green Assay using phosphate buffers or with biological samples that contain high levels of free phosphate. Remove free phosphate from samples by desalting column or dialysis. Storage and Stability: Store kit at 4 degrees C. Stable for 1 year.
Catalogue number: P4071-60
Size: 1Kit
References: 1. Harder, K.W., et al., Biochem. J. 298: 395-401 (1994). 2. Donella-Deana, A., et al., Biochim. Biophys. Acta 1094: 130-133 (1991). 3. Donella-Deana, A., et al., Biochim. Biophys. Acta 1051: 199-202 (1990). 4. Ek man, P. & Jager, O., Annal. Biochem. 214: 138-141 (1993).

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