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Cat no: P3123-83

Supplier: United States Biological
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3-Phosphoinositide-dependent protein kinase-1 (PDK1) is a serine/threonine protein kinase known to phosphorylate and activate PKB/AKT (Thr308 and Ser473), p70 S6 kinase, RSK, PKA, PKC and SGK. Upon stimulation of receptor tyrosine kinases, PI3K becomes activated and generates the phospholipid second messengers, PtdIns(3,4,5)P 3 and PtdIns(3,4)P 2.,which in turn mediate the phosphorylation and activation of PDK1 targets through diverse mechanisms (1-2). It has also been shown that stimulation of cells with pervanadate and IGF-1 results in a significant increase in PDK1 activity and translocation to the plasma membrane along with an increase in tyrosine phosphorylation (3). Only phosphorylation on the activation loop at Ser241 is necessary for PDK1s activity and a single nucleotide mutation abolishes its activity (4). Applications: Suitable for use in Western Blot, Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunohistochemistry: 1:100-1:250 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Manufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
Catalogue number: P3123-83
Reactivities: Human, Mouse
Hosts: Rabbit
Applications: Immunohistochemistry, Western Blot
Size: 100ul
Form: Supplied as a liquid in 50 mM Tris-Glycine, pH 7.4, 0.15 M sodium chloride, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
P type: Mab
Isotype: IgG
Purity: Supernatant
References: 1. Alessi et al. Curr. Biol. 7:776-789, 1997 2. Stokoe et al. Science 277:567-570, 1997 3. Jongsun P, et al. J. Biol. Chem. 276(40):37459-71, 2001 4. Casamayor A, Biochem. J. 342, 287-292, 1999.
Additional info: Recognizes human PDK1 phosphorylated on Tyrosine 373 and 376 as well as non- phosphorylated PDK1 . Species crossreactivity: mouse.

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