A convenient set of four small molecule modulators for enhancing neuronal transdifferentiation. The four products are: SB-431542 (Cat #S0098-93), LDN193189, CHIR99021 and Noggin, human recombinant.
SB-431542 (Cat #S0098-93): TGF-b1 receptor ALK5 inhibitor (1x1mg)
LDN193189: BMP type I receptors ALK2 and ALK3 inhibitor (1x1mg)
CHIR99021: GSK-3b inhibitor (1x1mg)
Noggin, human recombinant: Inhibits TGF-b signal transduction by binding to TGF-b family ligands. BMP4 antagonist. (1x20ug)
SB-431542, LDN193189 and CHIR99021 are soluble in DMSO (~20mg/ml) or Ethanol (~2mg/ml), DMSO (~4.5mg/ml) and DMSO (~46.5mg/ml) respectively.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with DMSO or ethanol. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.