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MAP Kinase, p38 (Thr180/Tyr182) BioAssay(TM) Phosphorylation Detection Kit

Cat no: M2352-06

Supplier: United States Biological
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p38 MAP kinase (MAPK), also called RK (1) or CSBP (2), is the mammalian orthologue of the yeast HOG kinase which participates in a signaling cascade controlling cellular responses to cytokines and stress (1-4). Four isoforms of p38 MAP kinase, p38alpha, beta, gamma (also known as ERK6 or SAPK3) and delta (also known as SAPK4) have been identified. Similar to the SAPK/JNK pathway, p38 MAP kinase is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharides (LPS), UV light and growth factors (1-5). MKK3, MKK6 and SEK activate p38 MAP kinase by phosphorylation at Thr180 and Tyr182. Activated p38 MAP kinase has been shown to phosphorylate and activate MAPKAP kinase 2 (3) and to phosphorylate the transcription factors ATF-2 (5), Max (6) and MEF2 (5-8). M2352-06: MAP Kinase, p38 (Thr180/Tyr182) BioAssay(TM) Phosphorylation Detection Kit will detect 20 Western Blot Assays. Kit Components: M2352-06A: Phospho-p38 MAP Kinase (Thr180/Tyr182), 1x200ul. M2352-06B: p38 MAP Kinase Pab, 1x200ul. M2352-06C: Control Cell Extract, Non-phosphorylated, 1x60ul. M2352-06D: Control Cell Extract, Phosphorylated, 1x60ul. M2352-06E: IgG (HRP) Pab GtxRb, 1x100ul. M2352-06F: Biotin (HRP) Pab Gt, 1x250ul. M2352-06G: Biotinylated Protein Ladder, 1x250ul. M2352-06H: LumiGLO Reagent, 20X, 1x10ml. M2352-06J: Peroxide, 20X, 1x10ml. Storage and Stability: Store all components at -20 degrees C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: M2352-06
Applications: Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Size: 1Kit
References: 1. Rouse, J. et al. (1994) Cell 78, 1027-1037. 2. Han, J. et al. (1994) Science 265, 808-811. 3. Lee, J.C. et al. (1994) Nature 372, 739-746. 4. Freshney, N.W. et al. (1994) Cell 78, 1039-1049. 5 Raingeaud, J. et al. (1995) J. Biol. Chem. 270, 7420-7426. 6. Zervos, A.S. et al. (1995) Proc. Natl. Acad. Sci. USA 92, 10531-10534. 7. Zhao, M. et al. (1999) Mol. Cell. Biol. 19, 21-30. 8. Yang, S.H. et al. (1999) Mol. Cell. Biol. 19, 4028-4038.
Additional info: Recognizes phosphorylated threonine 180 and tyrosine 182 of p38 MAP Kinase but does not appreciably crossreact with the corresponding phosphorylated forms of either p42/44 MAP Kinase or SAPK/JNK. Species crossreactivity human, rat, mouse, yeast.

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