p38 MAP kinase (MAPK), also called RK (1) or CSBP (2), is the mammalian orthologue of the yeast HOG kinase which participates in a signaling cascade controlling cellular responses to cytokines and stress (1-4). Four isoforms of p38 MAP kinase, p38alpha, beta, gamma (also known as ERK6 or SAPK3) and delta (also known as SAPK4) have been identified. Similar to the SAPK/JNK pathway, p38 MAP kinase is activated by a variety of cellular stresses including
osmotic shock, inflammatory cytokines, lipopolysaccharides (LPS), UV light and growth factors (1-5). MKK3, MKK6 and SEK activate p38 MAP kinase by phosphorylation at Thr180 and Tyr182. Activated p38 MAP kinase has been shown to phosphorylate and activate MAPKAP kinase 2 (3) and to phosphorylate the transcription factors ATF-2 (5), Max (6) and MEF2 (5-8).
M2352-06: MAP Kinase, p38 (Thr180/Tyr182) BioAssay(TM) Phosphorylation Detection Kit will detect 20 Western Blot Assays.
Kit Components:
M2352-06A: Phospho-p38 MAP Kinase (Thr180/Tyr182), 1x200ul.
M2352-06B: p38 MAP Kinase Pab, 1x200ul.
M2352-06C: Control Cell Extract, Non-phosphorylated, 1x60ul.
M2352-06D: Control Cell Extract, Phosphorylated, 1x60ul.
M2352-06E: IgG (HRP) Pab GtxRb, 1x100ul.
M2352-06F: Biotin (HRP) Pab Gt, 1x250ul.
M2352-06G: Biotinylated Protein Ladder, 1x250ul.
M2352-06H: LumiGLO Reagent, 20X, 1x10ml.
M2352-06J: Peroxide, 20X, 1x10ml.
Storage and Stability:
Store all components at -20 degrees C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.