Less selective than Macconkey Agar and will permit growth of Enterococci and Staphylococci.
MacConkey Agar without Crystal Violet is a modified version of the first media developed for the isolation of Gram negative enteric bacteria. The deletion of crystal violet allows the growth of enterococci, staphylococci and Mycobacterium sp. Gram negative enteric bacteria which ferment lactose will produce red to pink colonies. Because this medium is less inhibitory medium it is recommended that specimens be plated on other more inhibitory Gram negative media such as Deoxycholate Agar.
Quality Control Specifications:
1. The powder is homogeneous, free-flowing and light pinkish-beige.
2. Visually the prepared medium is clear to slightly hazy and reddish orange.
3. Expected cultural response after 18-24 hours at 35.0 degrees C.
Organism/Result:
Escherichia coli ATCC 25922
Growth, Pink to red colonies
Proteus mirabilis ATCC 12453
Growth, Clear colonies
Salmonella typhimurium ATCC 14028
Growth, Clear colonies
Enterococcus faecalis ATCC 29212
Growth, Pink to red colonies
Storage: Store the sealed bottle containing the dehydrated medium at 2-30 degrees C. Once opened and recapped, place the container in a low humidity environment at the same storage temperature. Protect it from moisture and light. The dehydrated medium should be discarded if it is not free flowing or if the color has changed from the original light pinkish-beige color.
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