Home  >  Products  >  JNK1/2/3, phosphorylated (Tyr185, Tyr185, Tyr223) (c-Jun N-terminal Kinase 1, JNK1, JNK1A2, c-Jun N-terminal Kinase 2, JNK2, JNK21B1/2, JNK2alpha, JNK2B, JNK2beta, c-Jun N-terminal Kinase 3, JNK3, JNK3A, JNK, JNK 46, JNK-46, JNK 55, JNK-55, MAP Kinase p49
JNK1/2/3, phosphorylated (Tyr185, Tyr185, Tyr223) (c-Jun N-terminal Kinase 1, JNK1, JNK1A2, c-Jun N-terminal Kinase 2, JNK2, JNK21B1/2, JNK2alpha, JNK2B, JNK2beta, c-Jun N-terminal Kinase 3, JNK3, JNK3A, JNK, JNK 46, JNK-46, JNK 55, JNK-55, MAP Kinase p49

JNK1/2/3, phosphorylated (Tyr185, Tyr185, Tyr223) (c-Jun N-terminal Kinase 1, JNK1, JNK1A2, c-Jun N-terminal Kinase 2, JNK2, JNK21B1/2, JNK2alpha, JNK2B, JNK2beta, c-Jun N-terminal Kinase 3, JNK3, JNK3A, JNK, JNK 46, JNK-46, JNK 55, JNK-55, MAP Kinase p49

Cat no: J2500-25

Supplier: United States Biological
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C-Jun N-terminal kinases (JNK) are distantly related to mitogen-activated protein kinases (ERKs) and are activated by dual phosphorylation on Tyr and Thr. The JNK protein kinase group consists of three isoforms (JNK1, 2, and 3) and regulates transcription activity of the AP-1 complex, and release and regulation of cytochome c (1). JNK becomes activated in vivo in response to pro-inflammatory cytokines or cellular stresses. Its full activation requires the phosphorylation of a threonine and a tyrosine residue in a Thr-Pro-Tyr motif, which can be catalysed by the protein kinases mitogen-activated protein kinase kinase MKK4 and MKK7 (2). Unlike other JNK isoforms, JNK3 is only expressed in heart and nervous system nervous. JNK3 has been implicated in stress-induced neuronal apoptosis (3). Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:5000-1:20,000 Immunoprecipitation: 1:20 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Manufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
Catalogue number: J2500-25
Reactivities: Human, Mouse, Rat
Hosts: Rabbit
Applications: Immunoprecipitation, Western Blot
Size: 100ul
Form: Supplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 40% glycerol, 0.01% sodium azide, 0.05% BSA.
P type: Mab
Isotype: IgG
Purity: Supernatant
Alternative names: 230008H04Rik, AI849689, FLJ12099, FLJ33785, MGC50974
References: 1. Kuan CY, et al. PNAS 100(25):15184-89, 2003 2. Fleming Y, et al. Biochem J 352(Pt1):145-54, 2000 3. Gupta S, et al. EMBO J. 15:2760-2770, 1996.
Additional info: Recognizes human JNK1 (pY185), JNK2 (pY185) and JNK3 (pY223). Species Crossreactivity: Mouse and rat.

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