c-Jun N-terminal kinase (JNK) is one of the several main MAP kinase groups identified in mammals. Recent evidences suggest that activation of JNK may play an important role in neuronal apoptosis and other physiological and pathological processes.
Sample Type:
Cell and tissue lysates.
Intended Use:
The kit specifically detects JNK, other kinase activity would not be detected.
Test Principle:
The JNK Activity BioAssay(TM) Kit utilizes a JNK-specific antibody to immunoprecipitate JNK from cell lysate. Activity of the JNK is then determined in a kinase reaction using recombinant c-Jun as substrate. Phosphorylation of the c-Jun can be analyzed by Western blot analysis using a phospho-c-Jun specific antibody.
Kit Components:
Kinase Extraction Buffer, 1x80ml
JNK Specific Antibody, 1x80ul
Protein A Sepharose, 1x2ml
c-Jun Protein/ATP Mixture, 1x80ul
Kinase Assay Buffer, 1x25ml
Phospho-cJun Specific Antibody, 1x50ul
Storage and Stability:
Store powder at 4 degrees C liquid at -20 degrees C. Store other components at 4 degrees C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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