Ionomycin was isolated from Streptomyces conglobatus as a potent antibiotic selective for Gram +ve bacteria. During the isolation it was recognised that ionomycin exhibits a very high affinity and selectivity for calcium ions suggesting the metabolite acts as a calcium ionophore. More recently, ionomycin has been used in cell biology as a universal calcium ionophore to explore the role of calcium regulation in the cell.
Soluble in ethanol, methanol, DMF or DMSO. Poor water solubility.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with buffer. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Toxicity and Hazards: