Home  >  Products  >  Interleukin 12 receptor, Beta 2 (IL-12-Rb2 , beta 2 IL-12R-beta-2, IL-12 receptor beta-2, Interleukin-12 receptor beta-2 chain ) (APC)

Interleukin 12 receptor, Beta 2 (IL-12-Rb2 , beta 2 IL-12R-beta-2, IL-12 receptor beta-2, Interleukin-12 receptor beta-2 chain ) (APC)

Cat no: I8434-50C

Supplier: United States Biological
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The biological activities of IL-12 are mediated through a high-affinity receptor complex composed of the IL-12 receptor beta 1 (IL-12 Rbeta1) and IL-12 receptor beta2 (IL-12 Rbeta2) subunits. IL-12 Rbeta2 is a 130kD protein that interacts with p35 and is associated with Jak2. The expression of IL-12 Rbeta2 is absent on nai?ve T cells. Activation of T cells via TCR upregulates IL-12 Rbeta2 expression on human Th1, but not Th2 cells. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 10ul labels up to 1x10e6 cells. Tested using human Th1-differentiated PBMCs. Briefly, PBMCs were treated with recombinant human IL-12 (5ng/ml) and anti-human IL-4 (10ug/ml) for 2 days, then activated with PMA (50ng/ml) plus ionomycin (200ng/ml) for 3 hours. Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Cells may be Fc-blocked with 1ug of human IgG/10e5 cells for 15 minutes at RT. Do not wash excess blocking IgG from this reaction. 2. After blocking, add 10ul of I8434-50C to up to 1x10e6 cells and incubate for 30 minutes at RT. 3. Remove unbound antibody by washing the cells twice in Flow Cytometry Staining Buffer (Catalog # F4568-10). Note that whole blood requires a RBC lysis step at this point using Flow Cytometry Human Lyse Buffer. 4. Resuspend the cells in Flow Cytometry Staining Buffer for analysis by flow cytometry. As a control for this analysis, cells in a separate tube should be treated with APC-labeled mouse IgG1 antibody. Storage and Stability: May be stored at 4 degrees C before opening. DO NOT FREEZE! Stable at 4 degrees C as an undiluted liquid. Stable for at 12 months at 4 degrees C. Freezing allophycocyanin conjugates will result in a substantial loss of activity. Allophycocyanin conjugates are sensitive to light.
Catalogue number: I8434-50C
Reactivities: Human, Mouse
Hosts: Mouse
Applications: Flow Cytometry
Size: 100Tests
Form: Supplied as a liquid in saline, 0.5% BSA, 0.1% sodium azide. Labeled with Allophycocyanin (APC).
P type: Mab
Isotype: IgG1
Purity: Purified by Protein G affinity chromatography.
References: 1. Trinchieri, G. et al. (2003) Immunity 19:641. 2. Brombacher, F. et al. (2003) Trends Immunol. 23:207. 3. Trinchieri, G. (2003) Nat. Rev. Immunol. 3:133. 4. Rogge, L. et al. (1997) J. Exp. Med. 185:825. 5. Shaojing, C. et al. (2007) Nat. Immunol 8:723.
Additional info: Recognizes human Interleukin 12 receptor, Beta 2. Species Crossreactivity: mouse

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