Interleukin 1, Family Member 6 (IL-1F6, FIL1e) (APC)

Human interleukin 1 family member number 6 [IL-1F6; also FIL-1E (epsilon)] is a recently discovered member of the IL-1 family of proteins. 1-3 IL-1 family members include IL-1b, IL-1a, IL-1ra, IL-18 and IL-1F5 through F10. 4 All family members show a 12 b-strand, b-trefoil configuration, and all family members are believed to have arisen from a common ancestral gene that has undergone multiple duplications. 4 IL-1F9 is synthesized as a 158 amino acid (aa) protein that contains no signal sequence, no prosegment and no potential N-linked glycosylation site(s). 1-3 It appears to be actively secreted. 1 When found in cell lysate, it presents as an 18 kD monomer. 2 Human to mouse, full length IL-1F6 has 54% aa identity. Within the family, IL-1F6 is 30% aa identical to IL-1ra, and 27%, 31%, 36%, 46%, 57% and 28% aa identical to IL-1b, IL-1F5, F7, F8, F9 and F10, respectively. Cells reported to express IL-1F6 include monocytes, B cells and T cells. 1,4 Notably, IL-1F6 is the only novel IL-1 family member found to be expressed on T-cells. The receptor for IL-1F6 is reported to be a combination of IL-1 Rrp2 and IL-1RacP. 5 Recombinant IL-1F6, along with IL-1F8 and IL-1F9, has been shown to act as an agonist by activating the pathway involving NF-kB and MAPK in an IL-1 Rrp2 dependent manner. This suggests that IL-1F6 may signal in similar fashion to IL-1 and IL-18 in having a binding receptor which upon ligation, recruits a second receptor as a signaling component, forming an active heterodimeric receptor complex. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C before opening. DO NOT FREEZE! Stable at 4 degrees C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Freezing APC conjugates will result in a substantial loss of activity. APC conjugates are sensitive to light.