The Immunohistochemistry Detection Kit is a reliable and convenient tool to identify specific gene expression on tissues or immobilized cells. Each kit contains all reagents, including buffer and chromogen, required for the immunohistochemistry. A biotinylated, cross-adsorbed, and affinity purified secondary anti-mouse IgG is used to detect primary antibody-antigen complexes adhered to a glass microscope slide, following reaction with an enhanced detection reagent. This kit is compatible with mouse IgG primary antibodies and can be used for both frozen and paraffin-embedded tissues.
Each kit contains all reagents, including normal goat serum, biotinylated anti-IgG, Streptavidin, Biotinlated HRP, liquid DAB, DAB buffer and detoxification buffer, for a whole procedure of immunohistochemistry. A simple protocol and recommended experimental conditions are provided. Each kit is enough for immunostaining 100 to 500 standard microscope slides and is stable for 2 years at 4 degrees C.
Simple to use:
A highly optimized protocol guides you through each step of the process of the IHC and the counterstaining. Straight-forward instructions, color-coded reagents, notes and trouble-shooting tips are valuable aids to the novice and the expert.
High signal with low background
In order to minimize DAB toxic disposal requirements, this kit has a special reagent to make DAB substrate non-toxic.
Can be used fot both frozen and paraffin embedded tissue sections
1. Components in this kit are free of precipitate.
2. Each lot is tested by p53 antibody.
100 Tests 500 Tests
I7506A: Normal serum 250ul 1.25ml
I7506B: IgG (Biotin) 250ul 1.25ml
I7506C: Solution A 260ul 1.3ml
I7506D: Solution B 260ul 1.3ml
I7506E: DAB Buffer 10ml 50ml
I7506F: DAB 250ul 1.25ml
I7506G: DAB Detoxification Reagent 1ml 5ml
Storage and Stability:
Store components at 4 degrees C. Stable for 6 months For maximum recovery of product, centrifuge the original vial prior to removing the cap.