This Immunohistochemistry Detection Kit, HRP (DAB, Broad Spectrum), BioAssay(TM) is a proprietary and enhanced HRP polymer technology designed to provide excellent sensitivity, high specificity, and a shorter protocol than conventional immunohistochemical staining methods. New polymer conjugation methods have resulted in a versatile polymer capable of intense nuclear, cytoplasmic, and membrane antigen staining. The polymer conjugate contains multiple HRPs to provide excellent sensitivity and specificity. The polymer staining method is faster and easier than a conventional LAB-SA protocol because it uses an HRP polymer conjugated to the second antibody and eliminates one incubation step. In addition, the kit does not contain biotin nor streptavidin, so potential background due to endogenous biotin activity is completely avoided. Another advantage is the shorter polymer incubation time; the system only requires 10 mintues, while leading competitors polymers require 30 minutes.
Reactivity:
Mouse, rabbit, rat, guinea pig
Applications:
Suitable for use in Immunohistochemistry (FFPE). Other applications not tested.
Kit Components:
I7506-07A: One dropper bottle (15ml) of ready-to-use HRP polymer conjugate.
I7506-07B: One dropper bottle (3ml) of 20X buffer/substrate
I7506-07C: One dropper bottle (3ml) of 20X DAB chromogen
I7506-07D: One dropper bottle (3ml) of 20X 0.6% H2O2
Reagents Required But Not Supplied:
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