Human IGF-BP3, also known as growth-hormone-dependant binding protein, is a 28.9kD cysteine-rich secreted protein. It is the major IGF binding protein present in the plasma of human and animals. In addition to its ability to modulate the activity of IGF-I and IGF-2, IGF-BP3 also exerts inhibitory effects on follicle stimulating hormone (FSH) activity. Decreased plasma levels of IGF-BP3 often results in dwarfism, whereas elevated levels of IGF-BP3 may lead to acromegaly. The expression of IGF-BP3 in fibroblasts is stimulated by mitogenic growth factors such as Bombesin, Vasopressin, PDGF, and EGF. Recombinant human IGF-BP3 is a 28.8kD protein consisting of 264aa residues.
Source:
Recombinant corresponding to human IGF BP3 expressed in E.coli.
Molecular Weight:
~29kD
Biological Activity:
The ED50 as determined by its ability to inhibit IGF-II induced proliferation of MCF-7 is ~0.2mg/ml, in the presence of 15ng/ml of human IGF-II.
Endotoxin:
~0.1ng/ul
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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