Hypoxia Inducible Factor 1 alpha (Hypoxia Inducible Factor 1 alpha Subunit Basic Helix-loop-helix Transcription Factor, HIF1A, HIF-1 alpha, HIF-1A, HIF1-alpha, ARNT Interacting Protein, Basic Helix-loop-helix PAS Protein MOP1, Member of PAS Protein 1, Mem

Cat no: H9804-04J

Supplier: United States Biological

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Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. HIF-1 is a nuclear protein involved in mammalian oxygen homeostasis. This occurs as a posttranslational modification by prolyl hydroxylation. HIF-1 is a heterodimer composed of HIF-1 alpha and HIF-1 beta subunits. Both subunits are constantly translated. However, under normoxic conditions, human HIF-1 alpha is hydroxylated at Pro402 or Pro564 by a set of HIF prolyl hydroxylases, is polyubiquinated, and eventually degraded in proteosomes. Under hypoxic conditions, the lack of hydroxylation prevents HIF degradation and increases transcriptional activity. Therefore, the concentration of HIF-1 alpha increases in the cell. In contrast, HIF-1 beta remains stable under either condition. HIF hydroxylases provide insight into hypoxic cell responses, which may be used to help isolate therapeutic targets. Applications: Suitable for Western Blot and Immunhistochemistry. Not recommended for Immunoprecipitation. Other applications not tested. Recommended Dilutions: Western Blot: 1:500-1:2000 Immunohistochemistry (Frozen/paraffin): 5-10ug/ml Optimal dilutions to be determined by researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Catalogue number:	H9804-04J
Reactivities:	Human, Mouse, Rat, Non-Human Primate
Hosts:	Rabbit
Applications:	Immunohistochemistry, Western Blot
Conjugates:	Biotin
Size:	100ul
Form:	Supplied as a liquid in PBS. No preservatives added. Labeled with Biotin.
P type:	Pab
Isotype:	IgG
Purity:	Purified by immunoaffinity chromatography.
References:	1. Ramanathan, M., et al., Mol. Biol. Cell 18(1): 14-23 (2007). 2. Bayele, H.K., et al., Blood 110: 3039-3048 (2007). 3. Kundu, C.N., et al., Carcinogenesis 28: 2089-2095 (2007). 4. Liao, D., et al., Cancer Res. 67: 563-572 (2007). 5. McLaren, A., et al., Am. J. Physiol. Regul. Comp. Physiol. 292: R403-R414 (2007). 6. Ream, M.A., et al., Am. J. Physiol. Regul. Comp. Physiol. 295(3): R942-R953 (2008). 7. Saini, Y., et al., J. Biol. Chem. 283(48): 33,650-33,657 (2008). 8. Wirthner, R., et al., Carcinogenesis 29(12): 2306-2316 (2008).
Additional info:	Recognizes mouse HIF-1 alpha. Species Crossreactivity: rat, human and primate. Antibody has been tested in Western Blot, recognizing a band in hypoxic samples at ~115kD. Rat retina and brain cortex have been tested. Rat brain cortex is a bit dirty, but this antibody does detect upregulation of HIF-1 alpha in hypoxic samples.

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Hypoxia Inducible Factor 1 alpha (Hypoxia Inducible Factor 1 alpha Subunit Basic Helix-loop-helix Transcription Factor, HIF1A, HIF-1 alpha, HIF-1A, HIF1-alpha, ARNT Interacting Protein, Basic Helix-loop-helix PAS Protein MOP1, Member of PAS Protein 1, Mem

Cat no: H9804-04J

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