It is expected that the His-Tag monoclonal antibody will react with proteins tagged with His from all species.
Applications:
Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested.
Recommended Dilution:
Western Blot: 1-2ug/ml
Immunoprecipitation: 10-20ug/ml
Immunocytochemistry: 10-20ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.