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Ghrelin, Rat, Mouse, BioAssay(TM) ELISA Kit

Cat no: G2033-73

Supplier: United States Biological
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This Enzyme Immunoassay kit is designed to detect a specific peptide and its related peptides based on the principle of "competitive" enzyme immunoassay. Plasma, serum, culture media, tissue homogenate, CSF, urine or any biological fluid can be assayed as long as the level of the sample is high enough for the sensitivity of the kit to detect. Plasma Extraction: Extraction is strongly recommended but not required. Peptide Sequence used in kit:� Gly-Ser-Ser(Octanoyl)-Phe-Leu-Ser-Pro-Glu-His-Gln-Lys-Ala-Gln-Gln-Arg-Lys-Glu-Ser-Lys-Lys-Pro-Pro-Ala-Lys-Leu-Gln-Pro-Arg Kit Components: G2033-73A: 96-well Microplate1x1plate G2033-73B: Assay Buffer (20X) 1x50ml G2033-73C: Serum 1x5ml G2033-73D: Standard1x1ug G2033-73E: Peptide (Biotin) 1x5ml G2033-73F: Streptavidin (HRP) 1x30ul G2033-73G: Substrate solution 1x12ml G2033-73H: Stop Solution, 2N HCI 1x15ml G2033-73J: Positive Control 2x200ul Sensitivity: Minimum Detectable Concentration: 0.1ng/ml Precision:� Intra-assay error: <5% Inter-assay error: <14% Range: 0-100ng/ml Linear Range: 0.10-1.05ng/ml Specificity: Peptide/% Crossreactivity Ghrelin (Rat) 100 Ghrelin (Human)100 Ghrelin (Des-Octanoyl-Ser3) (Human)100 Ghrelin (Des-Octanoyl-Ser3) (Rat) 100 C-Terminal of Ghrelin 100 Ghrelin (Porcine) 0 Ghrelin (1-14) 0 Ghrelin (1-5) 0 Secretin (Human) 0 VIP (Human, Porcine, Rat) 0 Prolactin-Releasing Peptide-31 (Rat) 0 Prolactin-Releasing Peptide-31 (Human) 0 Galanin (Rat) 0 Galanin (Human) 0 GHRF (Rat) 0 GHRF (Human) 0 NPY (Human Rat) 0 Orexin A (Human Bovine Mouse Rat) 0 Orexin B (Human) 0 Orexin B (Mouse, Rat) 0 beta-Endorphin (Rat) 0 beta-Endorphin (Human) 0 alpha-MSH 0 MCH 0 Storage and Stability: Store components at 4 degrees C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.�
Catalogue number: G2033-73
Reactivities: Mouse, Rat
Applications: ELISA
Size: 1Kit
References: 1. Enzyme Immunoassay Techniques, An Overview. Porstmann, T. and Kiessig, S.T. Journal of Immunological Methods, 150(1992) 5-21. 2. Amplification Systems in Immunoenzymatic Techniques. Avrameas, S. Journal of Immunological Methods. 150(1992) 23-32. 3. Shuto, et al. Journal of Clinical Investigation. 109 (2002) 1429-1436.

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