This Enzyme Immunoassay kit is designed to detect a specific peptide and its related peptides based on the principle of "competitive" enzyme immunoassay. Plasma, serum, culture media, tissue homogenate, CSF, urine or any biological fluid can be assayed as long as the level of the sample is high enough for the sensitivity of the kit to detect.
Plasma Extraction: Extraction is strongly recommended but not required.
Peptide Sequence used in kit:�
Gly-Ser-Ser(Octanoyl)-Phe-Leu-Ser-Pro-Glu-His-Gln-Lys-Ala-Gln-Gln-Arg-Lys-Glu-Ser-Lys-Lys-Pro-Pro-Ala-Lys-Leu-Gln-Pro-Arg
Kit Components:
G2033-73A: 96-well Microplate1x1plate
G2033-73B: Assay Buffer (20X) 1x50ml
G2033-73C: Serum 1x5ml
G2033-73D: Standard1x1ug
G2033-73E: Peptide (Biotin) 1x5ml
G2033-73F: Streptavidin (HRP) 1x30ul
G2033-73G: Substrate solution 1x12ml
G2033-73H: Stop Solution, 2N HCI 1x15ml
G2033-73J: Positive Control 2x200ul
Sensitivity:
Minimum Detectable Concentration: 0.1ng/ml
Precision:�
Intra-assay error: <5%
Inter-assay error: <14%
Range: 0-100ng/ml
Linear Range: 0.10-1.05ng/ml
Specificity:
Peptide/% Crossreactivity
Ghrelin (Rat) 100
Ghrelin (Human)100
Ghrelin (Des-Octanoyl-Ser3) (Human)100
Ghrelin (Des-Octanoyl-Ser3) (Rat) 100
C-Terminal of Ghrelin 100
Ghrelin (Porcine) 0
Ghrelin (1-14) 0
Ghrelin (1-5) 0
Secretin (Human) 0
VIP (Human, Porcine, Rat) 0
Prolactin-Releasing Peptide-31 (Rat) 0
Prolactin-Releasing Peptide-31 (Human) 0
Galanin (Rat) 0
Galanin (Human) 0
GHRF (Rat) 0
GHRF (Human) 0
NPY (Human Rat) 0
Orexin A (Human Bovine Mouse Rat) 0
Orexin B (Human) 0
Orexin B (Mouse, Rat) 0
beta-Endorphin (Rat) 0
beta-Endorphin (Human) 0
alpha-MSH 0
MCH 0
Storage and Stability:
Store components at 4 degrees C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.�
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