Beta-1, 4-galactosyltransferase 1 (B4GalT1) is one of seven B4GalT genes. Human B4GalT1 encodes a type II membrane protein, which is ubiquitously expressed and involved in glycoconjugate biosynthesis. A soluble form (aa78-398), derived from proteolytic cleavage, is a component of lactose synthase in milk that also contains alpha-lactalbumin. The amino acid sequence of human B4GALT1 is 88%, 87% and 71% identical to that of rat, mouse/canine and chicken.
Suitable for use in Western Blot, Immunoprecipitation and ELISA. Other applications not tested.
Western Blot: 1-2ug/ml The detection limit for recombinant human B4GaIT1 is ~5ng/lane under non-reducing and reducing conditions using a colormetric detection system.
Immunoprecipitation: 25ug/ml immunoprecipitates recombinant human B4GaIT1 from conditioned cell culture medium.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.