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FLI1, CT (Friend Leukemia Integration 1 Transcription Factor, Fli-1 Proto-oncogene, Transcription Factor ERGB)

FLI1, CT (Friend Leukemia Integration 1 Transcription Factor, Fli-1 Proto-oncogene, Transcription Factor ERGB)

Cat no: F6230-10E

Supplier: United States Biological
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FLI1 is a member of ETS family of transcription factors and is a proto-oncogene involved in erythroleukemia and Ewings sarcoma. FLI1 was originally identified as a common proviral insertion (Friend leukemia insertion site 1) and found in 75% of erythroleukemia cell clones induced by the Friend mouse leukemia virus. A chromosomal translocation between the FLI1 gene and EWS gene is found in most Ewing sarcomas. FLI1 has anti-apoptotic acivities and also interferes with nuclear hormone receptors. Applications: Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 2ug/ml Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml Immunoprecipitation: 2ug/mg lysate Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: F6230-10E
Reactivities: Human
Hosts: Rabbit
Applications: Immunohistochemistry, Immunoprecipitation, Western Blot
Size: 50ug
Form: Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.05% sodium azide.
P type: Pab
Purity: Purified by immunoaffinity chromatography.
Additional info: Recognizes human FLI1. Species sequence homology: Mouse and rat.

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