Fibroblast Surface Protein

Prolyl 4-hydroxylase (P4-H), an enzyme localized to the endoplasmi reticulum (ER), catalyses the co- and post-translational hydroxylation of proline residues in Xaa-Pro-Gly sequences of collagens and proteins with collagen-like aa sequences. Vertebrate P4-H consists of two distinct polypeptides; the catalytically more important, glycosylated alpha-subunit (64kD) and the beta-subunit (60kD) which belongs to the multifunctional enzyme protein disulphide isomerase (PDI). The beta subunit is a major cellular thyroid hormone-binding protein and shows partial homology with a phosphoinositide-specific phospholipase C, thioredoxins, and the estrogen-binding domain of the estrogen receptor.It catalyzes the formation, breakage and rearrangement of disulfide bonds. It also acts as a chaperone that inhibits aggregation of misfolded proteins in a concentration-dependent manner at high concentrations. Other function of the protein includes its ability to bind thyroid hormone, its role in both the influx and efflux of S-nitrosothiol-bound nitric oxide, and its function as a subunit of the microsomal triglyceride transfer protein complex. The COOH-terminus of the beta subunit has the aa sequence Lys-Asp-Glu-Leu, which was recently suggested to be necessary for the retention of a polypeptide within the lumen of the endoplasmic reticulum.\n\nApplications:\nSuitable for use in Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunohistochemistry (formalin fixed paraffin embedded): 1:25-1:50\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nThymus\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

F4213-60A

Size

500ul

Applications

IHC

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid, 0.05% sodium azide.

P Type

Mab

Purity

Ascites

Isotype

IgG1

References

1. Hoyntya et al. Eur J Biochem 141: 477, 1998. 2. Janin et al. Clin Exp Rheumatol 8: 237, 1990. 3. Koivu et al. J Biol Chem 262:6447, 1987. 4. David et al. Biochem J 317: 659,1996. 5. Kivirikko et al. The FASEB Journal 3: 1609,1989.

Additional Info

Recognizes proteins of 43kD and 72-80kD.Recognizes a surface protein on fibroblasts (synovial, foreskin and thymic), fibroblast cell lines, tissue macrophages, and 95% of peripheral blood monocytes. Does not stain lymphocytes or human epithelial cells but does stain malignant fibrosarcoma tissue.