Epstein-Barr virus (EBV) nuclear antigen 1 (EBNA1) is an EBV antigen that is expressed in all EBVassociated malignancies. EBNA1 is known to be required for maintenance of the EBV episome, and is expressed in all forms of EBV latency. Thus, EBNA1 is supposed to play a central role in the maintenance and segregation of the episomal latent EBV genome.
Applications:
Suitable for use in ELISA, Western Blotting or as an antigen in immunological protocols. Useful marker of chronic infection and useful in IgA and IgM studies. Other applications not tested.
Recommended Dilution:
ELISA: (same/less than) 1ug/ml for coating plates
Western Blot: 1-5ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degrees C or colder. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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