Cyclin A

Cyclins controls the transitions between the phases of the eukaryotic cell cycle as regulatory subunits of the cyclin dependentkinases (cdk). Human cyclins have been grouped into 5 types, A through E, based largely upon sequence similarity. Cyclin A first identified in sea urchin embryos is a 33kD protein identical to adenovirus e1a-associated protein p60. It can bind and activate the kinases p33cdk2 and p34cdc2. In addition to its mitotic functions, cyclin A is essential for progression through S phase. During S phase, cyclin A forms complex containing cdk2, the pRB-related protein p107, and the transcription factor E2F. It may indeed be directly involved in DNA replication; it colocalizes with the DNA replication complex and was found in a cellular activity that promotes large tumor-antigen-dependent simian virus 40DNA replication in Gl-phase cell extracts. It maps to 4q25-q31 region of human chromosome.\n\nApplications:\nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:100-1:500\nImmunohistochemistry (formalin fixed paraffin embedded): 1:20-1:40\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nHuman lung cell line\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

C8452-06B

Size

500ul

Applications

IHC, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid, 0.05% sodium azide.

P Type

Mab

Purity

Supernatant

Isotype

IgG1

References

1. Bodey et al. Modern Pathol 7: 846, 1994. 2. Inohara et al. Arch Dermatol Res 286: 504, 1994. 3. Dutta et al. Natl Acad Sci 92: 5386, 1995. 4. Berthold et al. Cell Biology 91: 5490, 1994.

Additional Info

Recognizes human Cyclin A.